Rational Design of Covalent Multiheme Cytochrome‐Carbon Dot Biohybrids for Photoinduced Electron Transfer
نویسندگان
چکیده
Biohybrid systems can combine inorganic light-harvesting materials and whole-cell biocatalysts to utilize solar energy for the production of chemicals fuels. Whole-cell have an intrinsic self-repair ability are able produce a wide variety multicarbon in sustainable way with metabolic engineering. Current biohybrid yet undefined electron transfer pathway between light-absorber enzymes, limiting rational design. To enable engineering efficient pathways, covalent biohybrids consisting graphitic nitrogen doped carbon dots (g-N-CDs) outer-membrane decaheme protein, MtrC from Shewanella oneidensis MR-1 developed. is subunit MtrCAB protein complex, which provides direct conduit bidirectional exchange across bacterial outer membrane. The g-N-CDs functionalized maleimide moiety by either carbodiimide chemistry or acyl chloride activation coupled surface-exposed cysteine Y657C mutant. MtrC∼g-N-CD characterized native denaturing gel electrophoresis, chromatography, microscopy, fluorescence lifetime spectroscopy. In presence sacrificial donor, visible light irradiation results reduced MtrC. may find application photoinduced transmembrane S. chemical synthesis future.
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ژورنال
عنوان ژورنال: Advanced Functional Materials
سال: 2023
ISSN: ['1616-301X', '1616-3028']
DOI: https://doi.org/10.1002/adfm.202302204